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anti flag m2 magnetic beads  (Bimake Inc)

 
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    Structured Review

    Bimake Inc anti flag m2 magnetic beads
    Anti Flag M2 Magnetic Beads, supplied by Bimake Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag+m2+magnetic+beads/anti+beads+flag+magnetic/pm41678333-283-8-12
    Average 86 stars, based on 1 article reviews
    anti flag m2 magnetic beads - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: MARK2 serves as a key regulator of host antiviral immunity through GEF-H1 phosphorylation.
    Article Snippet: A549 cells were transfected with GEF-H1-Flag and lysed 48 h post-transfection using Cell Lysis Buffer for Western and IP (Beyotime, P0013). .. GEF-H1-Flag was immunoprecipitated using Anti-Flag M2 Magnetic beads (Bimake, B26101). ..

    Magnetic Beads:

    Article Title: MARK2 serves as a key regulator of host antiviral immunity through GEF-H1 phosphorylation.
    Article Snippet: A549 cells were transfected with GEF-H1-Flag and lysed 48 h post-transfection using Cell Lysis Buffer for Western and IP (Beyotime, P0013). .. GEF-H1-Flag was immunoprecipitated using Anti-Flag M2 Magnetic beads (Bimake, B26101). ..

    Article Title: MARK2 serves as a key regulator of host antiviral immunity through GEF-H1 phosphorylation.
    Article Snippet: Protein concentrations were determined using BCA Protein Assay Kit (Pierce). .. 500 μg of total protein was incubated with anti-Flag M2 Magnetic beads (Bimake, B26101) for 1 h at 4◦C with rotation. .. Immunocomplexes were washed three times with RIPA-based wash buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, and 0.1% SDS) and eluted using sample buffer containing 1% SDS at 100◦C for 10 min. Proteins were subsequently separated by SDS-PAGE and transferred to PVDF membranes (Millipore) for immunoblot analysis using specific primary antibodies and HRP-conjugated secondary antibodies to mouse or rabbit.

    Incubation:

    Article Title: MARK2 serves as a key regulator of host antiviral immunity through GEF-H1 phosphorylation.
    Article Snippet: Protein concentrations were determined using BCA Protein Assay Kit (Pierce). .. 500 μg of total protein was incubated with anti-Flag M2 Magnetic beads (Bimake, B26101) for 1 h at 4◦C with rotation. .. Immunocomplexes were washed three times with RIPA-based wash buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, and 0.1% SDS) and eluted using sample buffer containing 1% SDS at 100◦C for 10 min. Proteins were subsequently separated by SDS-PAGE and transferred to PVDF membranes (Millipore) for immunoblot analysis using specific primary antibodies and HRP-conjugated secondary antibodies to mouse or rabbit.



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